FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30774365
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT65650.09.1200611
GGGGGGA328850.08.9397622
TACCGTA30550.08.7067329
CCGGGAT96400.08.330511
CGGGGAT101050.08.3233631
CGCGAGT33800.08.0134591
CCCGTAT44100.07.97361141
CCGGAAT70000.07.874481
CGGGGTT100350.07.86054371
GCGGAAT51700.07.7205931
CGGGTAT37800.07.6683151
GCGGGAT72000.07.5897461
CGTATCG31450.07.3982713
GATCGGA490950.07.371100494-95
CGCTAAT17551.0913936E-117.3105251
GGGGAAT170750.07.29125831
GCGTCGT204700.07.22008394-95
CGCGGTT39100.07.1702971
CGGGTGT95200.07.13774441
ATCGGAA464500.07.136054594-95
Produced by FastQC (version 0.11.5)