FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30362648
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT10751.8189894E-1210.1667131
CGCGGGT63650.09.3319591
CGCGAAT17700.09.1278151
GCGGGTT51650.08.556061
CCGGGAT82500.08.236511
GGGGAAT171500.08.0628781
CTCGTAT29200.07.97396761
GGGGGGA323450.07.8703522
CGAATCG16501.8189894E-127.7706943
GGCGAAT63800.07.74593541
CTCGGAT79850.07.7362351
CGGGCAT116800.07.60781571
GATCGGA516450.07.600803494-95
AATCCGC52900.07.54118165
GCGTCGT213050.07.450281694-95
CGGGGAT123300.07.43798731
CGGGGTT94950.07.4067551
ATCGGAA488300.07.338247394-95
CGGATCG20750.07.32338523
CGGGAAT67650.07.305111
Produced by FastQC (version 0.11.5)