FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17616836
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3902.2081678E-512.1829911
CGCGGGT38750.010.2997341
CGGGGAT76650.08.8642581
GGGGGGA166650.08.4922632
CGCGGTT21400.08.4370051
CGGCGAT29750.08.14521
GGCGAAT36650.08.0377811
CGGGGTT57000.08.0023011
GCGTCGT136650.07.99906394-95
GCGGGAT39100.07.89869071
AATCCGC28500.07.66497375
CGCGAGT18550.07.4280121
GGGGAAT92400.07.40472651
GATCGGA324750.07.38300194-95
AGCGTCG156150.07.365364694-95
CGGGCAT69050.07.36272531
CGCAAAT22700.07.32589441
AGATCGG356900.07.283861694-95
CGGGATT29600.07.22336051
GTCCGAT15804.838512E-107.2172651
Produced by FastQC (version 0.11.5)