FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15809749
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT54350.09.442471
CGCGGGT31600.08.8720971
CCGGGAT48550.08.6130081
GGCGAAT24950.08.5704451
CGCGGAT22500.08.4477061
CCGGTAT20550.08.3243831
CGGGTAT17150.08.3122481
TACCGTA14851.4551915E-117.99595079
GCGGTAT16051.8189894E-127.9937411
GCGGGAT37450.07.8668561
CGGGAAT37250.07.78152851
GGGGAAT86900.07.65543031
CTCGGGT42650.07.5761961
CGGCGAT30900.07.53527261
GCGGAAT26050.07.47889571
GTCGCAT21150.07.414211
GGGGGGA153350.07.37090642
CTACCGT19400.07.34471138
GCGTCGT108750.07.297486394-95
AATCGAC13053.8829967E-87.2781755
Produced by FastQC (version 0.11.5)