FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32248237
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT72750.08.9479421
CGGGGAT113050.08.9104761
CCGGGAT98700.08.9061531
CGGGATT66100.08.6261181
GGGGAAT175950.07.8314881
CGGGAAT76700.07.68178131
GCGTCGT220650.07.67820394-95
GGCGAAT51700.07.6282191
CGGGGTT109550.07.59034061
CTCGGAT90300.07.52460771
TCGGATT57300.07.459412
GATCGGA512700.07.45704594-95
GCGGAAT58350.07.3288731
CGACCTA22050.07.3222384
CTCGTAT34500.07.29948761
CGCGAGT36700.07.2503261
ATCGGAA495150.07.21267294-95
CGCGAAT21800.07.1927181
GTCGGAT36550.07.150081
AGATCGG575950.07.058935694-95
Produced by FastQC (version 0.11.5)