FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19105170
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA20450.09.2903829
CCGGGAT61100.09.0219371
GGGGAAT108500.08.8909731
GCGGAAT36600.08.4394721
CGGGGAT69700.07.7723991
CCGGAAT48600.07.72456171
GTTCGAT22150.07.7234591
CGGGAAT44950.07.71749161
CGCGAAT13651.4097168E-97.6590171
CGCGGGT43900.07.5773361
GCGTCGT127250.07.46977594-95
CCCGTAT28150.07.42775061
CTACCGT27000.07.38839638
CGCGTAA6450.00342607687.36755371
CGCGGTT26100.07.2828691
CGGAAAT49700.07.26674941
GCGGTAT21750.07.21004061
CTCGTAT20050.07.11033251
GTCGAAT20251.8189894E-127.0401071
GTCGATT16309.495125E-106.9969161
Produced by FastQC (version 0.11.5)