FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20416343
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT43000.09.9459561
CGGGGAT68750.09.4002351
CCGGGAT58250.09.0552321
TACCGTA19650.08.9437819
CGCGAAT12703.6379788E-118.60590651
GGGGAAT119100.08.4186641
GGCGAAT29400.08.081561
CGCGCAT15951.8189894E-128.0440651
CTACCGT25200.07.91643578
CCGGAAT47550.07.59513661
CGCGGAT25700.07.5809431
GGGAAAT177500.07.52281671
CTCGGAT56350.07.50530961
CGTATCG22400.07.41974973
GCGGAAT38200.07.339411
GCGTCGT143750.07.30637994-95
AGCGTCG167200.07.30490694-95
CGGGGTT71400.07.1878341
CGACCGT15301.8644641E-97.14346551
GATCGGA331350.06.99206494-95
Produced by FastQC (version 0.11.5)