FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31164288
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA384800.010.0099132
CGCGGGT64250.09.9835181
TACCGTA30050.08.3772689
CCGGGAT90550.07.9758711
CTCGTAT34600.07.96481
CGCGGAT41900.07.82452541
GGGGAAT173150.07.73837141
CGGGGAT102550.07.55222461
GATCGGA526800.07.50990394-95
GGGAAAT257400.07.4760031
CGGGAAT69750.07.357031
AGATCGG588500.07.328178494-95
GCGTCGT227850.07.27900994-95
CGGGGTT107650.07.19443231
CGGGTCT121750.07.0987083
CCGGAAT72000.07.06113051
AGAATCG79500.07.048423
ATCGGAA497800.07.01185294-95
CGCGTTA9556.768011E-56.96542551
GCGGGTT56850.06.93698261
Produced by FastQC (version 0.11.5)