FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20284981
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA18600.09.70391759
GGGGGGA250300.09.3544572
CGCGGGT41550.09.2632171
CGGGGAT66500.09.1461051
CGGGTAT25150.08.8798921
GCGGGAT46200.08.228031
GTCGGAT23250.08.1749451
GCGGAAT38250.07.82630831
CTACCGT24950.07.61492978
GCGGGTT39300.07.49629971
GATCGGA364350.07.408118794-95
ATCGGAA347050.07.359779494-95
CGGGGTT67150.07.35927721
CCGGGAT59050.07.2421991
GGGGAAT113950.07.21405741
GGGAAAT166550.07.13252351
AGAATCG52500.07.05528073
CGGGTTT46650.07.02821871
AGATCGG405350.07.01050694-95
CGGCAAC40800.06.98777531
Produced by FastQC (version 0.11.5)