FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21495208
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA286500.011.4053352
CGCGGGT46200.010.4910691
CCGGGAT61000.09.3478771
CGGGTAT23450.09.1186651
CGGGGAT66900.08.3814161
CGGGGTT70350.08.2405711
GGGGAAT125950.08.2246961
GCGGGTT38250.08.1992491
GTCGGAT24500.07.9520541
CGCGAAT12954.105459E-97.7056821
TACCGTA19750.07.69573939
GTTCGAT24550.07.54874321
GATCGGA414800.07.458636394-95
GGGGGAG333050.07.44266563
GCGGAAT39700.07.42100571
AGATCGG464050.07.373690694-95
AAAGGGG207250.07.28842980-81
CGGGAAT49400.07.2143281
CGCGGTT25450.07.09508131
TAAAAAA706000.07.07070170-71
Produced by FastQC (version 0.11.5)