FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39577555
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAT95800.08.9276871
GGGGGGA461750.08.9072392
CGCGGGT78900.08.7321841
GCGGAAT70600.08.4800381
TACCGTA37300.08.0224129
CGGGGAT131450.07.95232341
GGGGAAT219900.07.95160061
CGGCGAT69450.07.9362931
CGCGTAT10454.645717E-77.72973351
CGGGAAT91750.07.6127791
GGGAAAT329650.07.4951821
GATCGGA713200.07.31963894-95
CGGGCAT162050.07.30099871
GTTCGAT41200.07.2656631
GCCGATT43350.07.2341381
GCGTCGT307100.07.157028794-95
CTCGGAT102600.07.13190131
AGATCGG797450.07.03200894-95
CGTATCG44350.06.9596543
ATCGGAA676750.06.93792494-95
Produced by FastQC (version 0.11.5)