FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20585429
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT40600.011.4696861
GGGGGGA241850.010.1134522
CGCGTAA5203.6856713E-510.0517311
CGGGGAT66800.09.5319071
GGCGAAT28650.08.6244261
CTCGGTT37800.08.4223731
CGGGTAT21850.08.2638731
CGCGGAT26000.08.2241431
CGCGAGT22050.08.1889171
CGGGGTT68150.08.1577711
GGGGAAT114100.07.954251
CGGGATT37450.07.73979571
CCGGAAT46300.07.69718171
GGGAAAT178700.07.57831
GCGGTTT35150.07.57031871
CGGCAAT38200.07.4634451
CCGAAAC48550.07.4383371
CGGGCAT86600.07.2428171
CGCGAAT13204.741014E-87.19958641
GAATCGG33100.07.1732314
Produced by FastQC (version 0.11.5)