FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32517413
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT13400.010.638451
CGCGGGT66200.09.546751
GGGGGGA367350.09.2565462
CGGGGAT98900.08.60041
CCGGGAT94900.08.3620391
CGTATCG37000.07.9569243
GCGGAAT59200.07.9464911
TACCGTA30050.07.90318879
CGCGGAT41650.07.64401771
GGGGAAT186750.07.53171
GGGAAAT270350.07.4173371
GTCGAAA52000.07.40190651
CTCGGAT92750.07.3775211
CGGGGTT105650.07.37626031
GTATCGA43600.07.18841844
CCGGAAT73450.07.18113471
GCGGGAT72150.07.11294271
CTACCGT43100.07.0530378
CGACCTA18951.0913936E-117.0165684
GCCGGAT74800.06.98800131
Produced by FastQC (version 0.11.5)