FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21092232
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT52000.09.4124151
CGGGGTT68350.08.3427631
CGGGGAT69050.07.9829141
GGGGAAT118850.07.756571
CTCGGAT60450.07.70365671
CTCGGTT39000.07.6761441
CCGGAAT48600.07.62650351
CTCGACT32900.07.5105991
CGCGGGT46400.07.4760471
GGGAAAT172700.07.40162561
CGGGTAC33400.07.3981661
CGGCAAT43700.07.39425851
CGCGATT9103.5782694E-57.31061361
GCGGGAT52150.07.1984661
CGCAAAT31250.07.14685541
GGGGGGA255550.07.1363862
GATCGGA341800.07.118985794-95
TCGGATT36750.07.10768842
CGGGATT36750.06.9823821
ATCGGAA325150.06.96465194-95
Produced by FastQC (version 0.11.5)