FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n02_AHm301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n02_AHm301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20262002
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA19900.010.2635839
GGGGGGA239700.010.0058362
CTACCGT24200.09.42124758
CGCGGGT41200.09.22666551
GGGGAAT115950.08.6469641
CGGGATT36500.08.5921741
CGGGTAT23000.08.2638831
CTCGTAT22000.08.2075381
CGTATCG22950.08.0694183
CGGGGAT65050.08.03521251
CCGGGAT56850.07.6896991
GTATCGA27300.07.65363074
CGCAAAT30500.07.6339311
CGCGGAT26150.07.6318451
CGACCGT17601.8189894E-127.5595741
CGGGTCT77400.07.48478273
CGCGAAT10807.836079E-77.47957851
GCCGATT23800.07.38714651
GATCGGA297400.07.34269594-95
GTCCGAT19750.07.21782161
Produced by FastQC (version 0.11.5)