FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35838728
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424922011.856503389294398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC246900.036.5301068
TCGGAAG285750.032.518893
ATCGGAA293200.031.984082
CGGAAGA293050.031.7887344
GATCGGA289600.031.3092081
TCTCGTA74950.026.6583342-43
ACGTCTG171850.025.59109114-15
CTCGTAT72700.025.42232544-45
ACACGTC176050.024.99482512-13
CACGTCT175050.024.93335714-15
CGTCTGA177900.024.52065716-17
TATCTCG37200.024.26768340-41
ATGCCGT97600.024.21478548-49
CACACGT181950.024.14517612-13
GCACACG203100.021.68918210-11
GAGCACA449100.020.7915539
TGAACTC210700.020.6595920-21
CTGAACT215400.020.59415818-19
GAACTCC210000.020.51359220-21
GTCACGA42300.019.98881128-29
Produced by FastQC (version 0.11.5)