FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16056061
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT891800.5554288813426905TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG129400.042.05910544-45
CGTATGC132500.041.62847546-47
TATGCCG133350.041.46626348-49
CTCGTAT124000.040.59555444-45
TCTCGTA126700.037.2200942-43
GTCACGA153400.036.89536728-29
AGTCACG158350.035.83201628-29
CGAGATT161900.034.84083632-33
ATCTCGT134500.034.6200242-43
ACGAGAT165150.034.22710832-33
GAGCACA339350.034.0257579
TCACGAG167400.034.00852230-31
GTATGCC164300.033.70147346-47
ATCGGAA339250.033.6772232
AGAGCAC345900.033.3815468
ATGCCGT165350.033.29769548-49
CACGAGA171050.033.116230-31
TCGGAAG348000.032.912153
GATCGGA348700.032.3381651
CCGTCTT169900.032.065752-53
Produced by FastQC (version 0.11.5)