FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30774365
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1643330.5339931465685807TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC223300.041.475746-47
TCGTATG221150.041.18283544-45
TATGCCG228000.040.378748-49
CTCGTAT220750.039.98766744-45
TATCTCG228900.037.6209340-41
TCTCGTA236800.036.39685442-43
ATCTCGT260050.033.5081742-43
ACCGCTC285950.032.667332-33
GTATGCC287500.031.85872346-47
ATGCCGT286700.031.72204248-49
CGCTCAT299200.031.27671634-35
GTCACCG305900.030.2572928-29
CCGTCTT304400.030.01411652-53
GCCGTCT302050.029.90490750-51
GAGCACA643150.029.6806819
CACCGCT311550.029.655430-31
AGAGCAC656050.028.9739448
ATCGGAA666400.028.2577362
TGCCGTC329300.027.9567750-51
TCGGAAG678950.027.8052883
Produced by FastQC (version 0.11.5)