FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30362648
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG40250.036.23726340-41
GAATCGG131500.029.89683240-41
CACTCCG289800.029.11911830-31
AATCGGG131000.028.95919640-41
GAGCACA614100.028.130519
AGAATCG196900.028.01277738-39
ACTCCGG306800.027.65212432-33
CCGGAGA308050.027.30113434-35
ATCGCGT25800.026.97802242-43
TCCGGAG321700.026.51187134-35
CGGAGAA320050.026.21334636-37
AGAGCAC660300.026.004068
CTCCGGA328900.025.82296232-33
ATCGGAA671350.025.4236662
CGCGTAT14750.025.12331244-45
GTCACTC347300.024.54420528-29
ACGTCTG348750.024.49389514-15
TCGGAAG698850.024.4708983
GATCGGA699400.024.2078041
CGGAAGA707750.024.1901424
Produced by FastQC (version 0.11.5)