FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17616836
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT669170.3798468692107936TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG102350.039.6655344-45
CGTATGC105550.039.2714746-47
TATGCCG105450.039.1485248-49
CTCGTAT97100.037.9936744-45
TACTCGA116700.034.74532736-37
TCTCGTA102500.033.46745742-43
ACTCGAT114100.033.2678636-37
TTACTCG125600.033.01734534-35
ATCTCGT111750.030.2720542-43
GTATGCC139800.029.58227246-47
ATGCCGT147500.028.05228848-49
GCCGTCT146850.027.64198150-51
CTCGATC126450.027.14552138-39
CCGTCTT154900.026.37053552-53
TGCCGTC159800.026.04104650-51
ATTACTC164900.025.6381634-35
TCGATCT134400.025.25702338-39
ACATTAC177550.023.93179932-33
CACATTA177500.023.76462230-31
GAGCACA363000.023.5918969
Produced by FastQC (version 0.11.5)