FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15809749
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT566160.3581081521281584TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC89750.039.3540346-47
TCGTATG87250.039.14911744-45
TATGCCG92000.038.0283748-49
CTCGTAT85450.037.41635544-45
TCTCGTA93250.032.0212542-43
GTCACGA111350.031.90968128-29
AGTCACG119000.030.13776828-29
ACGAGAT120400.029.72739832-33
ATCTCGT99200.029.66958842-43
TCACGAG121700.029.60561230-31
CGAGATT122550.029.14772232-33
ATGCCGT119800.028.8865448-49
GTATGCC122500.028.67773246-47
CACGAGA125150.028.46685230-31
CCGTCTT128000.027.18169252-53
GCCGTCT127650.026.97953650-51
TGCCGTC140000.025.05761750-51
CATCTCG121150.024.13679540-41
GAGCACA309950.023.6149149
CGTCTTC150600.023.00801552-53
Produced by FastQC (version 0.11.5)