FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32248237
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1516240.47017764102887233TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG217150.040.9679844-45
CGTATGC223100.040.72538846-47
TATGCCG227200.040.0194448-49
CTCGTAT220300.038.8617944-45
TATCTCG224100.037.5464640-41
TCTCGTA234400.035.7758442-43
ATCTCGT256350.033.08320642-43
ACCGCTC289300.031.68175132-33
ATGCCGT288100.031.21366348-49
GTATGCC292400.030.9595746-47
CGCTCAT301900.030.48555634-35
CCGTCTT301900.029.79863452-53
GCCGTCT301550.029.4902350-51
GTCACCG311250.029.22655528-29
CACCGCT318200.028.6254130-31
GAGCACA677400.027.7597859
CCGCTCA330350.027.6730132-33
TGCCGTC330750.027.6263850-51
AGAGCAC687150.027.2830358
TCACCGC342250.027.02333830-31
Produced by FastQC (version 0.11.5)