FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm310.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm310.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19105170
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG22900.033.91980440-41
CACTCCG158200.029.11093330-31
GAATCGG74000.027.2210740-41
ACTCCGG174700.026.71488632-33
AATCGGG74550.026.31924840-41
CGGAGAA177100.025.91228936-37
AGAATCG114850.025.35639238-39
GAGCACA381750.024.9587239
AGAGCAC390600.024.3202618
CCGGAGA188750.024.22313334-35
TCCGGAG193450.024.17483334-35
ATCGGAA388600.024.1188892
TCGGAAG394950.023.8392943
ATCGCGT16900.023.61463542-43
GTCACTC200300.023.4664828-29
CGGAAGA406750.023.194484
GATCGGA400900.023.1841931
CTCCGGA201900.023.16290732-33
CGCGTAT9050.023.09825344-45
ACGTCTG206550.022.70776714-15
Produced by FastQC (version 0.11.5)