FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20416343
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1236300.6055442936083117TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG186800.042.2714144-45
CGTATGC190650.042.0137146-47
CTCGTAT173900.041.2543744-45
TATGCCG193900.041.1229348-49
TACTCGA200850.039.0856736-37
ACTCGAT190800.038.11928636-37
TCTCGTA175600.037.90747542-43
TTACTCG214800.037.45139734-35
ATCTCGT182100.035.9151442-43
ATGCCGT228400.034.7864848-49
GTATGCC230600.034.60118546-47
GAGCACA488550.033.8133939
AGAGCAC492000.033.5762838
GCCGTCT235100.033.52134750-51
ATCGGAA491700.033.2530862
CCGTCTT238900.033.2029352-53
TCGGAAG502400.032.6109773
CTCGATC202350.032.4704838-39
CGGAAGA511600.032.0989044
GATCGGA506700.032.089441
Produced by FastQC (version 0.11.5)