FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm308.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm308.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31164288
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1945790.6243652991526712TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC298450.042.42170746-47
TCGTATG291250.042.28938744-45
TATGCCG298300.042.23383348-49
CTCGTAT277950.041.44991344-45
TCTCGTA284500.037.50808342-43
GTCACGA348750.036.9055328-29
AGTCACG360100.036.1842228-29
ATCTCGT297850.035.7232442-43
TCACGAG367050.035.4733430-31
CGAGATT363200.035.260932-33
ACGAGAT368700.035.1729532-33
GAGCACA746350.034.9511389
ATGCCGT357200.034.90401548-49
AGAGCAC757300.034.3579648
GTATGCC366500.034.2404346-47
CACGAGA380750.033.77930-31
CCGTCTT371250.033.5335752-53
ATCGGAA771350.033.48372
TCGGAAG779900.033.2263643
GCCGTCT371150.033.07320850-51
Produced by FastQC (version 0.11.5)