FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20284981
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1387490.6839986687687802TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG189000.042.180644-45
CGTATGC193850.042.14060246-47
TATGCCG194200.041.7323948-49
CTCGTAT188250.040.6829744-45
TATCTCG190700.039.11403340-41
TCTCGTA198400.037.60894442-43
ATCTCGT206500.036.42132642-43
ACCGCTC234350.034.76220732-33
GTATGCC234750.034.4747346-47
ATGCCGT234500.034.19582448-49
GAGCACA493850.033.9607779
AGAGCAC499150.033.6192138
CGCTCAT244550.033.60403434-35
GCCGTCT237450.033.34978550-51
CCGTCTT238950.033.31516352-53
GTCACCG249950.032.35521328-29
CACCGCT251500.032.1839830-31
ATCGGAA520500.031.8882482
TCGGAAG526000.031.6360953
TGCCGTC259700.031.21504250-51
Produced by FastQC (version 0.11.5)