FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm306.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm306.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21495208
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1783640.8297849455562374TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC242800.042.9284646-47
TATGCCG244300.042.64290248-49
TCGTATG242200.042.03635844-45
TCGTATC232050.041.92951638-39
CTCGTAT234600.041.78818544-45
CGTATCT231700.041.03940238-39
TATCTCG235200.040.29876340-41
ATTCGTA263950.040.0635336-37
TTCGTAT256850.039.61736736-37
TCTCGTA243950.039.05909342-43
AATTCGT273150.038.693234-35
CACGAAT279800.038.26590730-31
GTCACGA283050.037.89357828-29
ACGAATT285150.037.88055832-33
ATCTCGT253600.037.7882642-43
GAGCACA580800.037.5202949
GAATTCG289800.037.0848134-35
AGTCACG291950.037.05567628-29
AGAGCAC595000.036.6966978
CGAATTC291200.036.6857432-33
Produced by FastQC (version 0.11.5)