FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm305.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm305.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39577555
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2287340.5779386826700134TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG324550.041.551844-45
CGTATGC332050.041.28402746-47
TATGCCG332900.041.13356848-49
CTCGTAT305900.040.27222444-45
TCTCGTA331550.036.65653242-43
AATCTCG334300.036.24021540-41
ATCTCGT352500.034.4442442-43
ATGCCGT404300.033.8340748-49
GTATGCC415700.033.0565746-47
CCGTCTT421250.031.892652-53
GCCGTCT420200.031.82357250-51
GAGCACA937250.030.9133349
AGAGCAC955350.030.1935318
TGCCGTC461650.029.61965650-51
ATCGGAA980000.029.1326262
TCGGAAG987800.028.993963
CGGAAGA1004250.028.5142974
GATCGGA1016550.027.9157031
CGTCTTC482100.027.87701252-53
GTCACAT522100.027.45410528-29
Produced by FastQC (version 0.11.5)