FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm304.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm304.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20585429
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1216850.5911220018781246TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC183550.041.683946-47
TATGCCG183850.041.50969748-49
TCGTATG179250.041.5064644-45
CTCGTAT172550.040.378544-45
TCTCGTA178150.036.337442-43
ATCTCGT183700.035.16197242-43
GTCACGA218950.035.12432528-29
AGTCACG224900.034.70197728-29
CGAGATT225050.034.04472432-33
TCACGAG230950.033.90595630-31
ACGAGAT229950.033.78404232-33
ATGCCGT222750.033.73814448-49
GTATGCC225750.033.6603446-47
CCGTCTT229700.032.7750452-53
GCCGTCT227650.032.6873250-51
GAGCACA483550.032.561589
AGAGCAC494400.031.9142388
CACGAGA241650.031.8542430-31
ATCGGAA503250.030.971692
TCGGAAG508600.030.701793
Produced by FastQC (version 0.11.5)