FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32517413
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1963150.6037226885176874TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC279350.041.29102746-47
TCGTATG274900.041.2702444-45
TATGCCG278800.041.19971548-49
CTCGTAT273950.040.01730744-45
TATCTCG280400.038.37832340-41
TCTCGTA291150.036.78264242-43
ATCTCGT308950.035.07096542-43
GTATGCC342300.033.48930446-47
ACCGCTC348500.033.2509732-33
ATGCCGT340650.033.2312248-49
CCGTCTT352300.032.276752-53
CGCTCAT365500.032.1465634-35
GCCGTCT353900.031.60388450-51
GTCACCG374700.030.86294728-29
CACCGCT376200.030.6260630-31
GAGCACA799200.029.8639879
TGCCGTC385450.029.76879950-51
AGAGCAC807900.029.4836928
CCGCTCA397850.029.27570532-33
TCACCGC406600.028.89118430-31
Produced by FastQC (version 0.11.5)