FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm302.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm302.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21092232
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG37700.036.1689140-41
CACTCCG224100.032.6637930-31
GAGCACA477550.031.697829
AGAGCAC482700.031.1630578
ATCGGAA490200.030.4681032
GAATCGG104750.030.41162740-41
TCGGAAG499100.030.114873
ACTCCGG244700.030.10808432-33
AGAATCG157100.029.99967238-39
CGGAAGA506300.029.6961354
GATCGGA503650.029.4676951
CGGAGAA247900.029.42595736-37
AATCGGG104800.029.21840940-41
CCGGAGA253200.028.90081234-35
TCCGGAG265050.028.03882234-35
CGCGTAT13750.027.98738344-45
ATCGCGT23750.027.7071542-43
CTCCGGA268600.027.61476132-33
GTCACTC278800.026.57020228-29
AAGAGCA571800.026.3153577
Produced by FastQC (version 0.11.5)