FastQCFastQC Report
Sat 20 Apr 2019
H7JN5BGXB_n01_AHm301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JN5BGXB_n01_AHm301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20262002
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1161880.5734280353935411TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG176450.042.02832844-45
TATGCCG180550.041.82115648-49
CGTATGC181200.041.80360846-47
CTCGTAT163400.040.83481244-45
TACTCGA192450.038.55774736-37
ACTCGAT183900.037.39257436-37
TTACTCG203250.037.16011434-35
TCTCGTA169700.036.85693442-43
GAGCACA441250.035.005969
ATCTCGT175200.034.95416342-43
ATGCCGT219200.034.12204748-49
GTATGCC220850.034.05108646-47
ATCGGAA449650.034.008452
AGAGCAC453550.033.9206438
TCGGAAG456100.033.589683
GATCGGA459050.033.198691
GCCGTCT225000.032.9778150-51
CGGAAGA466200.032.8724064
CCGTCTT228700.032.74170352-53
CTCGATC195550.031.70372638-39
Produced by FastQC (version 0.11.5)