FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39373325
Sequences flagged as poor quality0
Sequence length55
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32033268.135777204490603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT30800.06.9987261
GTCCTAA42700.06.82662631
GTCTATC41850.06.6726211
TAGACTG60900.06.43444975
GGTCTAC46250.06.35561
GTCTTAC34400.06.33750871
TCTATAC37700.06.30106163
CGTACTC48250.06.29409175
TATACTG56300.06.2641595
CTTATAC37450.06.21233843
TTAGACT42200.06.2096694
CTACACT52600.06.19245674
GTCCTAG49850.06.19145061
TAGGACT54450.06.16197354
CTATACT37000.06.15571264
GTCTATA45800.06.1506271
TACACTG71550.06.05860045
CTAGAAC59400.06.01935153
CTAGGAC56150.06.01883843
GTCTTAG50950.06.00970131
Produced by FastQC (version 0.11.5)