FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n02_DS_CCF_2_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n02_DS_CCF_2_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31655454
Sequences flagged as poor quality0
Sequence length55
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT32900.09.75806748
CGTGTAG107700.09.03279240
GCGTGTA124850.08.79189439
ATCTCGG46800.08.74492847
TCCTACG56600.08.26944742
TATACTG80650.08.0784545
GATCTCG44750.07.94082146
TTCCGCG161700.07.89440427
CGTACTC185400.07.8338143
GTCTATC57200.07.79308031
CGCGGAC189150.07.784454330
CGTGATC61400.07.78308747
GTCCTAT40050.07.76667261
CTAGGAC61000.07.6688793
CCTACGT57600.07.572894643
TCCGCGG186550.07.551645328
GGCGTGT144900.07.541494438
CTACGTG64000.07.46654544
TACGTAC89800.07.44585563
AGGCGTG184000.07.443832437
Produced by FastQC (version 0.11.5)