FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n02_DS_CCF_2_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n02_DS_CCF_2_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34689638
Sequences flagged as poor quality0
Sequence length55
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT38250.011.276257548
CGTGTAG144750.09.68313840
ATCTCGG51100.09.63954247
CTCGGTG55900.09.51347549
GTCCTAG53050.09.46484851
GCGTGTA166950.09.45130439
TACGTAC111600.08.9541863
GGCGTGT188500.08.70863538
GGTCTAC57700.08.5747361
GATCTCG52600.08.57260246
TCCTACG56350.08.47994742
TTCCGCG220750.08.31327527
GTCTATC61750.08.2106691
CAGGCGT236650.08.20005236
CCTACGT57700.08.19667543
CGCGTAT37150.08.17928442
AGGCGTG245900.08.15069437
CGCGGAC257850.08.14278230
TCCGCGG249500.08.08165528
CGTACTC259800.08.0509823
Produced by FastQC (version 0.11.5)