FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n02_DS_CCF_2_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n02_DS_CCF_2_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3811367
Sequences flagged as poor quality0
Sequence length55
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACG6600.012.99582142
GTCTATC7750.010.744711
CGTGATC8400.010.21113747
TATAGTG11150.09.8854165
TCTCGGT4401.7596903E-89.46863348
TATACTG8550.09.4537735
TAGTACC5701.8189894E-119.4537734
TACACTG12500.09.4056445
TCCTATA7400.09.2667332
CTACGTG8250.09.20834644
GTGCTAG8850.09.1324671
CGTGTAG11750.08.96794840
GTCTTAT5509.0039975E-108.9060421
ATCTCGG5001.4892066E-88.82242247
GATCTCG5102.0865627E-88.6496646
CTAGTAC5102.1091182E-88.6442143
GTCCTAC9700.08.58964941
TACGTGA9750.08.54604845
TGGTTCG11200.08.53090617
CCTATAC6056.020855E-108.5013343
Produced by FastQC (version 0.11.5)