FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n02_DS_CCF_2_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n02_DS_CCF_2_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6077214
Sequences flagged as poor quality0
Sequence length55
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGT6900.012.78681248
CTCGGTG10250.010.28142749
CCTATAC10150.09.8941853
GTCCTAT7900.09.6118621
ATCTCGG9700.09.348433549
TCCTATA11100.09.2677492
GCGTGTA30200.09.16759139
CGTGTAG26800.09.05185840
CTTAGAC11000.08.6842873
CAGGCGT39000.08.48104438
TTAGACT10400.08.4787414
CCTTTAG8700.08.4464641
GATCTCG9000.08.44138546
TAGACTG13400.08.4085755
GTTCTAG9350.08.1212531
GTCTATC9400.08.0780541
AGGCGTG43400.08.01645539
TCTGTAC18050.08.0063953
GGCGTGT34550.07.942370438
TCCGCGG46300.07.884185330
Produced by FastQC (version 0.11.5)