FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39373325
Sequences flagged as poor quality0
Sequence length20
%GC70

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGGGGGGGGGGGGGGGG35800389.092546794054096No Hit
ACGGGGGGGGGGGGGGGGGG10833992.751606576279753No Hit
TCGGGGGGGGGGGGGGGGGG7177071.822825478925135No Hit
CCGGGGGGGGGGGGGGGGGG6937001.761852726433442No Hit
GAGGGGGGGGGGGGGGGGGG6810501.7297243755766116No Hit
TGGGGGGGGGGGGGGGGGGG3716520.9439182492207605No Hit
GGGGGGGGGGGGGGGGGGGG3593880.9127702575284156No Hit
CGGGGGGGGGGGGGGGGGGG588990.1495911254637499No Hit
AGGGGGGGGGGGGGGGGGGG494430.12557486572444668No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGGG1404650.012.8824991
CGGGGGG8081200.011.9686152
TCGGGGG1033750.010.9731471
GCGGGGG5237450.010.8428461
GAGGGGG1038550.010.1405251
CCGGGGG1212700.09.4872621
AGGGGGG1254600.09.0755962
GGCACAA26400.07.7694131
GGACTAA20050.07.68126631
GGTCCCA36750.07.5242681
ATACGTC21050.07.48215638
GGACTTT30300.07.41629361
GGTACCA22500.07.3737831
GGACTAC19850.07.37072661
GGCCTAA31000.07.24882941
GGGATTC46150.07.23554371
GGACAAT21400.07.2294121
TGTTAAA21600.07.12971166
CTAAGAT22400.07.12507253
TACACAT29150.07.107881514
Produced by FastQC (version 0.11.5)