FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n01_DS_CCF_2_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n01_DS_CCF_2_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31655454
Sequences flagged as poor quality0
Sequence length20
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG1071750.3385672497383863No Hit
GCGGGGGGGGGGGGGGGGGG1037050.3276054736096977No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTA80300.011.5155994
CTGTTAA82900.011.4753225
TTAAATG81850.011.2633548
TGTTAAA81950.011.07023056
TACTGTT82400.010.8313253
GTTAAAT85200.010.812227
CCTATAA56900.010.6292386
CTTCCAT56350.010.6087083
CTCGTAC48300.010.5652816
TTATTCA70750.010.507454
CTATAAG57650.010.3694847
ACGCGTA43800.010.2603368
CATCCAC34600.010.2168256
CTGTCCA47400.010.1160624
GACATAA47150.010.036021
GGCTTAT66900.09.9297111
GGTTAAC36150.09.9142111
CGTAACT36800.09.9103387
CCATATC41850.09.9020745
CTTACGC48650.09.8993245
Produced by FastQC (version 0.11.5)