FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n01_DS_CCF_2_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n01_DS_CCF_2_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34689638
Sequences flagged as poor quality0
Sequence length20
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG1041080.30011267341561765No Hit
GCGGGGGGGGGGGGGGGGGG986820.2844711149767547No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC56300.010.4440898
CTAAGAT59000.010.0136253
TACACAT119600.09.91453814
CTTCGCC68550.09.864477
CTTCTAA51350.09.4060993
TAAGATA59300.09.0776194
TCTTCGC50300.08.9484076
AGTCCTA34500.08.8261198
ACCAAAT47250.08.8000464
TCTTCGA35750.08.7917046
TTCTAAT53400.08.7697084
GAACTCA29000.08.7621011
GGTCTTC65100.08.634441
TTCGCCT49550.08.6317178
ATACACA145000.08.57851713
GAAACCA28400.08.5774971
CAGCAGA36000.08.5750573
TACCAAA47350.08.5597193
GGACTAC26350.08.5275461
TCTAGTC39600.08.5202464
Produced by FastQC (version 0.11.5)