FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n01_DS_CCF_2_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n01_DS_CCF_2_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3811367
Sequences flagged as poor quality0
Sequence length20
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG177680.4661844424848093No Hit
GCGGGGGGGGGGGGGGGGGG144040.3779221470931558No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAT6200.010.27426055
TACGTTA6200.010.1613563
TCTACCC5500.09.9273383
GGCAATA4100.09.9023741
ACGTTAA5850.09.6923714
GACACCA3200.09.6249361
GAACACC3500.09.5999371
TAATCGA6350.09.5906148
TACACAT8150.09.36165614
CCCGTAA5500.09.290978
CGGACCC4300.09.2791315
CGCAAAC4250.09.223593
GAGACCT2900.09.1723541
ACTCACC2450.09.1431577
GGTCTAC4750.09.1367821
TTAATCG7450.09.1143937
GGCATTA4400.09.0681231
GAGAAAC2950.09.016891
ACTTAGT5700.08.8421638
GGAAATC1905.456968E-128.8420481
Produced by FastQC (version 0.11.5)