FastQCFastQC Report
Sat 4 May 2019
H7JM2BGXB_n01_DS_CCF_2_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JM2BGXB_n01_DS_CCF_2_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6077214
Sequences flagged as poor quality0
Sequence length20
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG200520.3299538242359081No Hit
GCGGGGGGGGGGGGGGGGGG170070.27984862800618837No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTAC20950.012.1288147
TCGATCA15300.011.9869633
CAGGATT15300.011.9411138
CGAATTT17800.011.8371143
ATCAGGA15400.011.7273076
CAAAGCC8950.011.65366655
TGTAGCT22150.011.629835
CAAGCCA11400.011.4824915
TCAGGAT15950.011.4105987
AGCTACT21450.011.3892178
ATGTAGC21700.011.3871314
TGCCAAT15100.011.3576493
TAGTTTT33500.011.3044188
TATTTTA34850.011.2884133
AAGCCAT10750.011.2651496
TTAGTTT33550.011.2458427
AGCCATA12650.011.2331417
GCCAATA16150.011.1826954
TCCTGCT20400.011.1519933
CAGTTAC10950.011.1233214
Produced by FastQC (version 0.11.5)