FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39589882
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG731976818.488986655731885No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC704040.17783331609828995No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA576270.14555991856707226No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG566340.14305170194748243No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA418250.10564567987345858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT474000.069.164747
TGAGGTA883800.068.962891
GTAGTAG838050.068.873695
GAGGTAG926550.068.823592
TGTATAG611250.068.8119214
GGTAGTA845000.068.795884
TAGTAGG475050.068.7948766
GTTGTAT487700.068.7570912
TATAGTT549700.068.7568116
TAGGTTG476100.068.6629
TTGTATA616950.068.2664813
TAGTAGT227350.068.0180366
TAGTTAA542500.067.93146518
GTATAGT618100.067.81711615
AGGTAGT856200.067.582963
ATAGTTA548850.067.42911517
CAGTGGC149700.067.3704455
GACTGAT417700.067.33710511
CAGACTG416300.067.295179
GGCATAG108250.067.1727213
Produced by FastQC (version 0.11.5)