FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9835567
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1134201.153161785182288No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA857780.872120539669955No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA636400.6470394640186987No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG610420.6206251251198838No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAACTGTAGGCACCATC535280.544228919390209No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT416800.4237681467677461No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC375900.3821843722888574No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG361780.3678283112707178No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA337370.3430102199496989No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG333300.33887217686585835No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC326640.3321008336377557No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAACTGTAGGCACCAT264240.2686576178068839No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC254140.25838876396246396No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC251860.25607064646095135No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAACTGTAGGCACCAT250320.25450490042922796No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT241030.24505958832876643No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA236090.2400370004088224No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA216270.21988564563690127No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC209620.21312446959082276No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA203340.2067394792796389No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTAACTGTAGGCACCAT180960.18398532590952815No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC179920.18292793897901363No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTGAACTGTAGGCACCATC173930.17683779694653087No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG167370.17016812553867No Hit
CCCCCACAACCGCGCTTGACTAGCTTGCTGTTTAACTGTAGGCACCATCA153200.15576122861040953No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA151170.15369729065950138No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA148620.15110465924333594No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG147140.14959991630375757No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT146270.14871537146765407No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC137630.13993092619876416No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT134740.13699261059377665No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA126050.12815732941476582No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG118560.12054211007865637No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTTAACTGTAGGCACCA103980.1057183586874046No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGAACTGTAGGCACCATC103600.10533200577048582No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG99920.10159048278558826No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA99520.10118379550462114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT433150.069.3386468
AGTAGGT435650.069.165677
CAGCACG242800.069.157444
GTTGTAT446550.069.1402412
TAGGTTG433950.069.075129
TAGTAGG436200.068.9664846
TTGGCGA220150.068.94530517
AGCTTAT423950.068.9371642
TGAGGTA840350.068.932971
TGTATAG572100.068.8451514
GGTAGTA784000.068.832844
GAGGTAG867200.068.791182
GTTGACA261150.068.7629918
GCTTATC424400.068.748623
GTAGTAG781100.068.7389145
CGTAAAT245100.068.7379769
AGGTTGT515400.068.7111410
GCACGTA244800.068.649616
TATAGTT511850.068.64754516
ATTGGCG238800.068.63154616
Produced by FastQC (version 0.11.5)