FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8428773
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC787900.9347742548055334No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG677830.8041858524366476No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA663880.7876354007872794No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG463070.5493919458977007No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT430420.5106555841520468No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA394400.46792101293984306No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC373470.4430894034042677No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307260.3645370447157611No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT299230.355010153909709No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC274630.32582441121619954No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC261420.3101519046722459No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA219570.26050054972414133No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC212900.2525871796523646No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA203820.24181455592646758No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC200770.23819599839739425No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG191070.22668779904263647No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT185540.2201269389981199No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT164600.1952834653394984No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA140130.16625195624558878No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG117840.13980682597573812No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG110430.13101551079854684No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG109690.1301375656931323No Hit
CTACGGGGATGATTTTACGAACTGAACTCTCTCTTTCTGATGGATTAGTG105770.12548682945904463No Hit
TGCAAATGATGCATATGTTAGCGACCAAAGCCTGATCTTTGCTGATTAGT104470.12394449346304617No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATC97400.11555655846942371No Hit
AGTAGTGATGAAATTCCACTTCATTGGTCCGTGTTTCTGAACCACATGAT92280.10948212746979898No Hit
GCTTAATGATGACTGTTTTTTTTGATTGCTTGAAGCAATGTGAAAAACAC89060.10566187984894125No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA87470.10377548428460465No Hit
CGTCCATGATGTTCCGCAACTACCTACATTGTTTGATCCTCATGAAAGCA84690.10047725807777715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT490900.069.486428
CAGCACG290050.069.450874
AGTAGGT493950.069.340767
GTTGTAT500900.069.3087112
GAGGTAG1024250.069.263042
TAGTAGG493500.069.227936
GTAGTAG927600.069.205315
TGTATAG644400.069.18461614
TAGGTTG492700.069.169469
TTGGCGA265850.069.15295417
TGCTGGC100350.069.1215449
GGTAGTA934900.069.087934
GCACGTA291750.069.046186
TATAGTT576550.069.02991516
ATTGGCG286200.068.9678916
AGATGCT100200.068.9460146
TGAGGTA1002750.068.941011
CAGTGGC180550.068.908355
GTATAGT645450.068.87308515
ACAGTGG180700.068.8705144
Produced by FastQC (version 0.11.5)