FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10113048
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC910700.9005198037228737No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG741860.7335671698581872No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA588460.58188194103301No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG406060.401520886680257No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT370550.36640783273252536No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG345280.34142031166073766No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC306550.3031232522578752No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA295600.29229565606729047No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC255710.25285156364332495No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT214550.21215166782556555No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA206830.20451796530581087No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC196970.19476818462643508No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG193670.1915050734457109No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC180560.1785416226641068No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC180500.1784822933699118No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT154790.15305969080736095No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG149190.14752229001582906No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG129020.12757775895061507No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT126770.12535291041830318No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT121650.12029014398033115No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA121350.11999349750935623No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA113080.11181594312614752No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA109950.10872093161230917No Hit
TTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCG105660.10447888707736779No Hit
ATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCCGCGACGG104220.10305498401668814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGGT529650.069.3860247
GTAGGTT527550.069.3766258
CAGCACG314150.069.261064
TAGGTTG528350.069.238469
GTTGTAT538750.069.1938912
GAGGTAG1042250.069.186732
TTGGCGA280550.069.1207117
TAGTAGG529550.069.111076
TGAGGTA1014350.069.051231
AGCTTAT515150.069.024492
CGTAAAT316600.068.976619
GGTAGTA951700.068.966664
TATAGTT601650.068.9578516
TGTATAG674450.068.95544414
GTAGTAG945000.068.937485
ATTGGCG309000.068.8493316
GCTGGCA125000.068.8453410
ACAGTGG197900.068.811464
GCACGTA316550.068.7915656
AGGTTGT618050.068.7758110
Produced by FastQC (version 0.11.5)