FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9683027
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG825680.852708559007426No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC704650.7277166530672692No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA665250.6870268976839576No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG436450.4507371506864537No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT411920.42540416338816367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378430.3908178713123489No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA286900.29629164516426526No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC232860.24048265072481984No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC202040.20865376085391477No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT195650.20205458479047927No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC181130.18705927392332997No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC179470.18534493397570823No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA159530.16475219990608309No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG156770.16190185155943487No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG142100.1467516304560547No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA136610.14108191581000445No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT132440.13677541124278597No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA110860.11448899192370321No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT109950.11354920315723585No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT98830.10206519097798654No Hit
TACAATGATGATAACATAGTTCAGCAGACTAACGCTGATGAGCAATATTA97580.10077427234272919No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG288200.069.266164
GTAGGTT460450.069.218788
GTTGTAT478700.069.20447512
AGTAGGT463200.069.19287
TAGGTTG461500.069.030959
GAGGTAG925450.069.003972
TGTATAG608700.068.988614
TAGTAGG462800.068.973886
TGAGGTA899200.068.92381
GGTAGTA838350.068.901034
TTGGCGA263150.068.8779717
CGTAAAT291850.068.818889
GTAGTAG834200.068.7613455
TATAGTT532350.068.7588316
GTTGACA306850.068.7537118
AGCTTAT535050.068.732362
GCTTATC534050.068.716893
ATTGGCG285100.068.7058416
AGGTTGT546050.068.6801710
GCACGTA291750.068.6632466
Produced by FastQC (version 0.11.5)