FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9415725
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG783980.8326283955829211No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG637330.6768783072997565No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA616630.6548938079648673No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC543990.5777462702022415No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG396720.4213377089921382No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT374150.3973671703453531No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA264720.28114669874067055No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC193330.2053267273630018No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT179920.19108459518518223No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC173530.18429807582528163No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC167920.17833995788959425No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG141880.1506840949581684No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC138050.1466164315546599No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA125360.13313897761457563No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA118660.12602322179120568No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT114190.12127584439859916No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG108910.11566820398854044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT458550.069.3908168
CAGCACG291850.069.310984
AGTAGGT460650.069.30247
GTTGTAT473150.069.2762812
TAGGTTG460100.069.180249
TTGGCGA265600.069.1508217
GAGGTAG914550.069.037452
TGTATAG604850.069.0050714
TAGTAGG462300.068.9346546
TGAGGTA889950.068.9339141
TATAGTT529400.068.92979416
GGTAGTA831850.068.896424
GCACGTA293750.068.88656
ATTGGCG286850.068.8716716
AGATGCT123350.068.831236
AGGTTGT541000.068.8164110
CGTAAAT294400.068.793489
GCTTATC550750.068.774443
AGCTTAT552150.068.7391362
GTAGTAG827900.068.722095
Produced by FastQC (version 0.11.5)