FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH222.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH222.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7817151
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG665400.8512052536787379No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA576460.737429787399527No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC414020.5296302962549911No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT375450.4802900698732825No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG360360.4609863619111362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293560.3755332345505415No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA286060.3659389462989777No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC226890.29024640818630726No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT200970.25708854798890285No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC197460.252598421087171No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC144980.18546398809489545No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA141680.18124250126420738No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG139280.17817232902370697No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT117280.15002908348578659No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT116280.14874984505224473No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC111650.14282697110494605No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC110520.14138143167504374No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA107670.13773560213944952No Hit
TATCTGTGATGATCTTATCCCGAACCTGAACTTCTGTTGAAAAAAAAAAA81730.10455215717337428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT407550.069.4777458
CAGCACG245900.069.367654
TAGGTTG408050.069.349739
AGTAGGT410250.069.2678767
GTTGTAT423650.069.23254412
TTGGCGA222600.069.1466517
TATAGTT482300.069.1244416
GAGGTAG831800.069.118732
TAGTAGG410350.069.081756
TGTATAG545100.069.07532514
ATTGGCG242150.069.0124116
TGAGGTA809350.069.0008241
AGGTTGT480500.068.9878710
GTAGTAG754700.068.974125
GGTAGTA759750.068.943624
GCTGGCA103600.068.91199510
AGATGCT103600.068.778196
AGCTTAT398350.068.76392
AGTAGTT213450.068.730517
CGTAAAT249100.068.71459
Produced by FastQC (version 0.11.5)