FastQCFastQC Report
Thu 18 Apr 2019
H7JJVBGXB_n01_AH221.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7JJVBGXB_n01_AH221.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9359699
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG740870.7915532326413488No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC600760.6418582477919429No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA597610.6384927549486368No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT376380.40212831630589824No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373250.398784191671121No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG351360.37539668743620924No Hit
GTTTGTGATGACTTACATGGAATCTCGTTCGGCTGATGACTTGCTGTTGA246760.2636409568299151No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC189960.2029552446077593No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT176880.1889804362298403No Hit
TGCTCTGATGAAATCACTAATAGGAAGTGCCGTCAGAAGCGATAACTGAC168100.17959979268564086No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC158650.1695033141557223No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC139140.14865862673575295No Hit
CTTCGTGATCGATGTGGTGACGTCGTGCTCTCCCGGGCCGGGTCCGAGCC125570.1341602972488752No Hit
ATACATGATGATCTCAATCCAACTTGAACTCTCTCACTGATTACTTGATG122820.1312221685761476No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG122060.13041017665204832No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT118420.12652116269978342No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA118350.12644637396993216No Hit
GTGCAATGATGTATTTTATTCAACACATCATTCTGAAAGAACGTGTGGAA114140.12194836607459278No Hit
CATATATGATGACTTAGCTTTTTTCCCCGACAGATCGACTATGTTGATCT99370.10616794407597938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT480800.069.426758
AGTAGGT483750.069.3578647
GTTGTAT500850.069.3380512
CAGCACG310400.069.239844
GAGGTAG985000.069.156742
TAGGTTG482650.069.124759
TGTATAG644400.069.07197614
TGAGGTA957800.069.0500261
TAGTAGG484450.069.012776
AGGTTGT569200.068.9557810
TTGGCGA282200.068.95425417
TATAGTT572800.068.9389716
GGTAGTA896000.068.9172064
CGTAAAT313100.068.9106459
AGATGCT123800.068.8927466
GTTGACA328800.068.8684618
GTAGTAG891050.068.868015
ATTGGCG305450.068.8616816
AAGATGC123950.068.837615
AGCTTAT541750.068.8065952
Produced by FastQC (version 0.11.5)